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Post by lebmung on May 21, 2020 17:50:11 GMT -5
Here is an overview paper on seedless citrus. Seedlessness in Citrus (A review).Has anyone tried chemical mutagens to induce seedlessness? I know they are dangerous for own health to work with, and have at hand one  I want to try to make a new seedless Yuzu. |
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till
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Posts: 160
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Post by till on May 31, 2020 15:41:55 GMT -5
Nice overview, the article. But I am in general not a friend of producing genetically illnesses as sterility is one. Couldn`t you try another method to obtain seedless fruits? I mean, you could choose self-incompartible cultivars that also produce parthenocarpic fruits. By self-incompartible I mean cultivars that have fertile pollen but do not accepts their own pollen like Clementine or pumelos. My knowledge is too limited what combinations would be possible. I thought of crossing Yuzu with Clemyuz or Sanford Curafora with anything else. Sanford Curafora produces parthenocarpic fruits and seems to be self-incompartible. But I do not know what feature Clemyuz has inherited from Clementine. Another idea: what would be crossong Satsuma (parthenocarpic) with Pumelo (self-incompartible)?
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kumin
Full Member
SE Pennsylvania, 45 miles north of Chesapeake Bay, Zone 6b
Posts: 113
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Post by kumin on May 31, 2020 16:49:53 GMT -5
Hybridization with any tetraploid cultivar should produce seedless triploids. By sorting large numbers of Yuzu seeds it should by possible to select for tetraploid, as well as triploid seeds. Small, undersized, incompletely filled seeds have a higher frequency of triploid and tetraploid seeds. By germinating the select seeds and testing ploidy, it should possible to find what you're looking for. By selecting directly for triploids, vs tetraploids a generation of time could be saved. This is only going to work if the selection is capable of producing parthenocarpic fruits. Perhaps Yuzu could be tested for parthenocarpy by emasculating flowers prior to anthesis, then bagging immediately to exclude any chances of pollination. If Yuzu isn't capable of setting unpollinated flowers, it may not be a good candidate for seedlessness.
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Post by ilya11 on May 31, 2020 17:27:36 GMT -5
Yuzu is not a parthenocarpic variety, but seedless Yuzu does exist.
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Post by lebmung on Jun 1, 2020 18:10:18 GMT -5
Yes, indeed seedless Yuzu exists.
I can make tetraploid Yuzu very easy with the help of a mutagen, don't need to have a large population. The problem would be waiting time to fruit then cross with a diploid and then wait again to fruit the new hybrid, that means 10-15 years.
I am looking for something faster. Gamma radiation would be the best, but I who has access to that.
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Post by martweb on Feb 8, 2022 1:33:05 GMT -5
Not every triploid outcome is seedless. The triploid Kumquat Reale for example contains seed.
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Post by Sylvain on Feb 8, 2022 9:14:31 GMT -5
> Has anyone tried chemical mutagens to induce seedlessness?
Yes I tried with Oryzalin, it works very well.
I mean the mutagenesis works well. Each treated bud shows abnormal growth.
But then it is very hard to keep them alive because other buds grow much faster and you would spend your time cutting "good" buds to keep the treated ones alive.
I didn't give up. One day I shall try again, but in better conditions.
P.S. If you don't have a good education in chemistry, don't try to do it.
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Post by radoslav on Apr 10, 2023 12:31:31 GMT -5
Yes, indeed seedless Yuzu exists. I can make tetraploid Yuzu very easy with the help of a mutagen, don't need to have a large population. The problem would be waiting time to fruit then cross with a diploid and then wait again to fruit the new hybrid, that means 10-15 years. I am looking for something faster. Gamma radiation would be the best, but I who has access to that. Trying to create tetraploid to get triploid in hobby conditions is nonsense, first you do not know, if the plant is real tertraploid and you will not be able to save triploid embryo anyway. |
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